FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49247035
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC103350.017.6351814
GTCCGTT33550.017.313171
CAGACCG107200.017.2537083
GACCGCG110700.016.484615
ACCGCGT116550.015.7704236
AACGCTA148150.014.83747423
ACGCTAC147450.014.80134224
CCGCGTT125400.014.6209787
CCGGCTT192650.014.5764252
CCCGTAT35650.014.46191
GTTCCTA74750.014.32557715
CGCTACC151450.014.29158325
GCGGACT74800.014.2286922
TCACGCT163200.014.2257738
CGCGTTC130900.014.0238378
TTCGACG21200.014.0078546
CCCGGAT102950.013.9522331
CTCGTAT58750.013.9489951
TTCACGC167700.013.884297
CCCCCCT39350.013.7886491
Produced by FastQC (version 0.11.5)