FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49247035
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG162500.013.75334645
GTTGTCG19300.013.40621426
GGGAGGT260550.013.1767113
GGCATAG181350.012.28442235
GGAGGTC285900.012.03175454
ATAGGGG186500.012.02978238
GAGCTTA200600.011.21962844
TGGCATA206050.010.95363634
GGTCACT316750.010.6392497
GTCACTC317600.010.469118
ACTCTCC333500.010.11833511
CATAGGG223750.010.03711737
TAGGCTA71550.09.904098510
GCATAGG235250.09.62292836
CGTAGGG108400.09.34167437
TCACTCT364700.09.2835659
GAGGTCA400150.09.2374135
ATCCTAG92000.09.2186826
TCTCCCC363500.09.20922613
TATCCTA81400.09.2035515
Produced by FastQC (version 0.11.5)