FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53909126
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1388560.2575741999601329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT252550.025.24870945
GACCGCG298450.021.7334945
ACCGCGT299300.021.6947826
AGACCGC306200.021.2863224
CAGACCG309500.021.16843
CCGCGTT310250.020.935627
CGCGTTC310600.020.8975438
AGAACGA307900.020.76132644
TCAGACC358300.018.4862792
AATACGG347650.018.35584832
TACGGAG347250.018.35023734
ATACGGA349550.018.2618533
GCGTTCT360000.018.1611759
AAGAACG357550.017.91101643
CAATACG362900.017.64012731
TTCGGCG69050.017.43346244
CTCAGAC418600.017.0515121
ACGGAGA381050.016.71667335
TCGGCGC73650.016.52765545
CGGAGAG386100.016.48132336
Produced by FastQC (version 0.11.5)