FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53909126
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG675840.12536652885079236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT349400.014.4898983
GGTCACT349300.013.9609497
GGAGGTC373050.013.4990314
GTCACTC383850.012.80450258
ACTCTCC415350.011.89989311
GAGGTCA430550.011.8583065
TCACTCT420600.011.7982599
TGGGAGG321200.011.2096432
AGCTTAG305050.011.13849145
ATAGGGG304800.011.1204138
GTTGTCG21300.011.09235226
GGCATAG304000.011.08976735
CACTCTC450000.010.87278810
TGGCATA322250.010.57336134
ACCCGGG102800.010.3914592
GAGCTTA331750.010.18198844
TCTCCCC493400.010.05886513
GCTCTGT493950.09.97569722
CTGGGAG417000.09.8374871
CTCTGTC516250.09.69304223
Produced by FastQC (version 0.11.5)