FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50399922
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1129720.2241511405513683No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG658010.1305577417361876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG54600.027.7754486
TCGACGA63800.023.8004937
CGGTATA89150.022.81756820
GTTCGAC65800.022.7087125
CGACGAG66800.022.2584638
GACCGCG296150.021.2763235
AGACCGC299650.021.0878894
ACCGCGT299800.021.0145576
GAACGAT300600.020.93214445
CAGACCG313850.020.327373
CGCGTTC313800.020.0426168
CCGCGTT315650.019.9409037
TCAGACC351200.018.3898282
AGTTCGA84850.018.3263724
GTATAGA122650.017.84537922
CAGTTCG92800.017.556623
ATGACGG110500.017.43476516
GCGTTCT367500.017.187399
ACGAGGA110350.017.14519910
CTCAGAC411950.016.732191
Produced by FastQC (version 0.11.5)