FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50399922
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1066670.21164120055582625No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG537430.10663310153535555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT444300.015.58956053
GGAGGTC446900.015.1967414
AGCTTAG362300.014.13421845
AGGTCAC489400.014.0653526
GGTCACT481200.014.0571887
GAGGTCA492300.014.0054075
GTCACTC490400.013.8714668
TGGGAGG393400.013.4081042
GTTGTCG31350.013.34900526
ACTCTCC519450.013.18901311
CTGGGAG435750.012.8022491
ATAGGGG400400.012.75548938
GGCATAG398950.012.67777735
TCACTCT546000.012.5894499
CACTCTC546100.012.54151110
GAGCTTA414250.012.27472244
AACTCTA130850.012.25980945
TGGCATA414050.012.24260134
GCTCTGT625600.010.90097222
CATAGGG473950.010.82824437
Produced by FastQC (version 0.11.5)