FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48898118
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1143410.23383517541513563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT207150.027.35673745
AGACCGC258000.022.0792434
AGAACGA258350.021.9612844
CAGACCG269600.021.2571583
GACCGCG276150.020.538195
ACCGCGT288550.019.6788026
CCGCGTT289000.019.6014527
CGCGTTC294000.019.2834018
AAGAACG295300.019.2366643
AATACGG296700.018.97762332
ATACGGA296450.018.96254533
TACGGAG296450.018.9245734
CAATACG310350.018.23047831
TCAGACC327200.017.8446392
GCGTTCT324300.017.6072679
CTCAGAC362300.017.3624861
ACGGAGA328300.017.19114335
CCGTACG59300.016.6172664
CCCAATA344600.016.57999629
CGGAGAG339150.016.56149336
Produced by FastQC (version 0.11.5)