FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48898118
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG127300.014.10346338
AGCTTAG134550.013.54475845
GGCATAG137600.012.78572635
GGGAGGT231850.011.7082133
GAGCTTA156900.011.6438244
GGTCACT228700.011.3416027
CATAGGG159300.011.22802937
GTCACTC237450.010.9807238
TGGCATA163100.010.92464634
GCATAGG170300.010.48912736
GGAGGTC261750.010.3924624
ACTCTCC252150.010.385076511
TCACTCT277400.09.6257629
AGGTCAC297800.09.4282666
GAGGTCA303850.09.3299535
GTTGTCG19550.09.32220926
GCTCTGT295950.08.82682922
TAGGGGA288500.08.75758439
ACACTCG81600.08.6580144
CACTCTC303750.08.57607210
Produced by FastQC (version 0.11.5)