FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59046995
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT115500.017.4719076
AGACCGC119000.017.0167034
GTCCGTT43750.016.516331
CCGCGTT123850.016.3300297
CGCGTTC124700.016.236768
CAGACCG128700.015.9089963
GACCGCG127600.015.816915
TTCGGCG86800.015.70993444
TCGGCGC91100.014.74609545
CCCCCCT50600.014.5918371
CCCGTAT44550.014.3501781
GAACGAT143300.014.27377345
GCACTAC80300.013.92646725
CGTACGC100450.013.90982155
CCGTACG100650.013.8821814
ACGTTCG21800.013.72546
AACGCTA113950.013.48563923
ATGCGCG26200.013.31225245
ACGCTAC116000.013.2670624
CTCCGAT55000.013.2198491
Produced by FastQC (version 0.11.5)