FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43193644
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG457850.1059993919475745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC100250.019.436174
TTCGACG14400.018.1244626
CAGACCG113900.017.3249383
GACCGCG122300.016.0054915
CGTTACG18250.015.53531521
ACCGCGT126650.015.5265836
CGTACGC77550.015.4639925
CCGTACG77600.015.3960724
GCCGGCT175900.015.2742161
CCCGTAT27050.015.223141
CCGCGTT134000.014.708527
GTCCGTT33650.014.6446661
GAACGAT122300.014.6070945
TCACGCT196250.014.4339678
CTCCGAT40250.014.4236191
CGCGTTC137150.014.4199158
TTCGGCG82900.014.41151544
TCGGCGC82800.014.37457445
TACGCCA84100.014.3663997
CCGGCTT218050.014.3228412
Produced by FastQC (version 0.11.5)