FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43193644
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG161250.013.69049745
GTTGTCG18150.013.63330226
GGCATAG160550.013.5351935
ATAGGGG164400.013.4375138
GGGAGGT222400.013.25328353
GGAGGTC238500.012.1977784
GAGCTTA182250.012.10044844
TCAGACG88600.012.021222
TGGCATA186850.011.78648134
CATAGGG199850.011.09903937
ACCCGGC55050.011.0420345
GCATAGG204150.010.82093236
GGTCACT263050.010.6329467
GTCACTC275100.010.3303598
ACTCTCC277800.010.14089511
TGGGAGG232700.010.0637092
TCACCCG71000.09.7998353
CGTGGCG144900.09.6975951
CAGACGT109200.09.6090823
GTAACGA36250.09.55738218
Produced by FastQC (version 0.11.5)