FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44775179
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG666320.14881459212033524No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT656730.14667278047062637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCTT252500.017.7315272
AGACCGC189300.017.7208254
TTCGACG13000.017.4791326
TCACGCT236600.017.0397118
GACCGCG198050.016.9151865
GCCGGCT227150.016.8929421
CAGACCG198600.016.8795743
TTCACGC247550.016.50417
GTCCGTT39450.016.4397011
ACCGCGT206900.016.3433236
GTTCCTA73750.016.25916915
ACGCTCA260500.015.98577710
CGGCTTC288950.015.6972053
GAACGAT212600.015.5895245
CCGCGTT217750.015.53927
CGCGTTC225900.014.9586588
CCTACTC78850.014.95080918
GCGGACT98700.014.474752
ACGCTAC271750.014.38952124
AACGCTA271600.014.34763523
Produced by FastQC (version 0.11.5)