FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44775179
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG711750.15896083854851814No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA561910.12549586903940685No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG487500.10887728667706721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT352700.014.8434493
GTTGTCG25750.014.15474526
GGAGGTC379050.013.7581744
AGCTTAG257900.013.56120645
GGTCACT386650.012.9518017
GTCACTC393600.012.7173888
GAGGTCA431700.012.2780085
GGCATAG285700.012.16048135
ATAGGGG288650.012.09887738
TGGGAGG302550.012.0691292
ACTCTCC426750.011.87714811
TCACTCT433800.011.7100029
GAGCTTA303800.011.65286644
TGGCATA311800.011.178555534
CACTCTC462700.010.82787610
TGTCGTT34650.010.71399128
ATATAGC38850.010.6549116
CTGGGAG373700.010.6502381
GCTCTGT501050.010.26970822
TCTCCCC501600.010.21708513
Produced by FastQC (version 0.11.5)