FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50060054
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1223510.24440844590379388No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG750560.14993191976980288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG26800.026.5287176
AGACCGC251000.025.0176054
CAGACCG259650.024.2968123
GAACGAT264950.023.40460845
GACCGCG298550.021.055585
CGGTATA42300.020.58383620
GCCGGCT183350.020.2626611
TCGACGA36150.020.1023927
GCGTTCT317150.019.9127489
CTCAGAC349950.019.8114381
GTTCGAC35550.019.6824265
AGAACGA317650.019.57848744
ACCGCGT330650.019.1478276
CGACGAG35700.019.0953648
TCAGACC340600.018.9846882
TACGGAG323350.018.91338234
ATACGGA331300.018.78556333
CCGCGTT337550.018.742647
CGCGTTC339550.018.6521198
AATACGG335500.018.56445532
Produced by FastQC (version 0.11.5)