FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50060054
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG524410.10475617944798862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT245550.014.9663533
AGCTTAG177100.014.91680545
ATAGGGG184150.014.4709838
GTTGTCG27850.014.13921226
GGCATAG184850.014.12277235
GGTCACT266700.013.2158757
ACTCTCC272100.013.11302111
GTCACTC272000.013.0334238
GAGCTTA202800.012.97229244
CATAGGG216600.012.24069637
GGAGGTC302800.012.1665054
CACTCTC293000.012.1614110
TCACTCT302550.012.0968199
TGGCATA224500.011.81892634
GTTCTAT140900.011.7636991
GAGGTCA317950.011.7355075
GCATAGG226000.011.73059636
AGGTCAC310500.011.7058926
TCAGACG80650.011.2373452
TGGGAGG262350.010.9978712
Produced by FastQC (version 0.11.5)