FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48993800
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT788970.16103466152860157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC171900.022.2263764
GAACGAT180600.020.95411945
CAGACCG184650.020.850073
GACCGCG189150.020.1159295
ACCGCGT192650.019.8536626
CCGCGTT198500.019.2685557
CGCGTTC200300.019.0841668
AGAACGA214250.017.72606544
TCAGACC225950.017.4126872
TTCGACG15650.016.9640056
GCGTTCT228650.016.8557039
AATACGG225500.016.80185332
TACGGAG227200.016.58697734
ATACGGA229800.016.53641133
CTCAGAC270000.016.038321
GTCCGTT34650.015.8655751
AAGAACG243350.015.6433543
AACGCTA130200.015.6215623
ACGCTAC132050.015.43678124
CGTTACG22550.015.26552821
Produced by FastQC (version 0.11.5)