FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48993800
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAT185350.010.6141361
ACCCGGC63200.010.5775415
ATAGGGG97850.010.37014938
TCTATAG237200.09.6600793
GGCATAG102250.09.6160935
AGCTTAG106850.09.56018245
GTTGTCG19550.09.4370826
CACCGAA230100.09.36771645
ATAGCTC66800.09.12785539
CTATAGT254950.09.0581414
TTCTATA267550.09.010292
GCACCGA240500.08.95331644
GTAACGA43250.08.94793318
ATAGTGT264250.08.9231866
GGGAGGT201300.08.9117323
TTACTAG255000.08.84107914
CATAGGG113200.08.82491937
GCGTAGG60200.08.78308936
TATAGTG265650.08.7356295
GGCGTAG60550.08.695250535
Produced by FastQC (version 0.11.5)