FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47018858
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG31450.026.3253366
AGACCGC156000.022.1976014
GACCGCG157450.021.9503085
ACCGCGT158500.021.831016
TCGACGA37650.021.8109537
CCGCGTT166300.020.8747127
CGCGTTC170000.020.473278
CAGACCG171100.020.3832653
CGGTATA52700.020.32211720
CGACGAG39950.019.9920548
GAACGAT178450.019.54164145
CGTTACG22950.018.92113721
ATACGGG69600.018.4582233
TCAGACC204400.017.4218252
TACGGGG76350.017.03248434
AACGACG28550.016.7061216
GCGTTCT214250.016.3813489
AATACGG214900.016.30110232
GTTCGAC50700.015.8879215
AGAACGA228950.015.30991144
Produced by FastQC (version 0.11.5)