FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57119628
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT843130.14760775402808995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT197400.020.26560845
AGACCGC217450.018.159784
TTCGGCG87100.017.69487444
CAGACCG228050.017.5426253
GACCGCG239600.016.6030525
ACCGCGT248350.016.1358666
AGAACGA252200.015.98703644
CGTACGC98650.015.715015
CCGCGTT255900.015.6949547
CCGTACG98200.015.580824
GTTCCTA94550.015.37253915
CGCGTTC262400.015.3233078
CCCGTAT40000.014.8573781
TTCGACG20650.014.4918586
GTCCGTT42300.014.0495311
TACGGAG288700.014.03596834
ATACGGA289550.014.01029133
AATACGG288150.013.97685432
TCAGACC295600.013.830132
TACGCCA112500.013.7803057
Produced by FastQC (version 0.11.5)