FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45556269
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT751750.16501570837594273No Hit
CTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCGCTGGGATAGAA463040.1016413350267995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT154850.022.20830345
AGACCGC168650.020.3778254
CAGACCG179200.019.3421823
AGAACGA185450.018.60736344
CCGTACG86300.017.707784
CGTACGC88400.017.490455
ACCGCGT207300.016.7003156
ATACGGA206350.016.6907633
TACGGAG206100.016.66723434
TACGCCA94200.016.4772037
TCCGTAC94150.016.4471593
AATACGG211050.016.26837332
GACCGCG217650.015.8725355
GCGTTCT219000.015.7978349
GTACGCC98800.015.7783526
CCGCGTT220600.015.7138457
CGCGTTC220950.015.6787728
CAATACG223900.015.43809131
TCACGCT148050.015.4372768
AAGAACG225050.015.40299543
Produced by FastQC (version 0.11.5)