FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45556269
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG553710.12154419406031691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG181000.015.4380145
GGGAGGT286400.014.360633
GGCATAG199350.014.03064835
GAGCTTA206700.013.57260644
ATAGGGG207450.013.57002438
GGAGGTC319250.012.678624
TGGCATA223100.012.65752734
CATAGGG236100.011.999577537
TGGGAGG262550.011.5454262
ACTCTCC329950.011.54077211
GCATAGG247700.011.51001536
GGTCACT345950.011.3636977
GTCACTC336300.011.3551678
TCAGACG79700.011.2377442
TGTCCAA368100.010.7763626
TAGCGTA6700.010.74759837
TCTCCCC355500.010.72358313
GCTCTGT371700.010.64485922
CACTCTC362250.010.62596710
AGCATAG102600.010.57785945
Produced by FastQC (version 0.11.5)