FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52594517
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG173700.014.107532545
ATAGGGG183750.013.60690438
GTTGTCG27100.013.53311926
GGCATAG183200.013.38982435
GGGAGGT290250.012.4227753
GTCACTC288500.012.1491038
GAGCTTA209200.011.93961944
ACTCTCC301900.011.63255711
CATAGGG214000.011.51539137
TGGCATA216150.011.38002834
GGAGGTC320150.011.2843524
AGGTCAC321400.011.0587016
GAGGTCA325900.011.0439165
GGTCACT317250.010.9200957
GCATAGG227350.010.82919536
CACTCTC336850.010.38538610
TCACTCT345550.010.3516529
TGGGAGG283500.09.5444142
GCTCTGT371200.09.48599922
TTACGCG10950.09.45404235
Produced by FastQC (version 0.11.5)