FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49385648
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG749070.1516776695934009No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG669370.13553937775606387No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA539770.10929693582232636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA48800.020.56110814
GCGTCGT49750.020.21364411
GTCGTGT51700.019.62517513
GGTCGCC59700.017.52539343
TGTAGGG62100.017.42702517
GTAGGGA63600.015.70708718
AGTGTAG66950.015.5600428
GTCGCCG73000.015.07213144
GTAGATC72000.014.90617431
GTTGTCG32950.014.88599526
TCTCGGT70550.014.7344136
ATCTCGG77450.014.64191235
GGGAGGT365350.013.9834543
CGTCGTG73100.013.60301112
AGCTTAG273250.013.43843445
TAGATCT77400.013.37217432
AGCGTCG79050.013.26160810
GGAGGTC374500.013.1312114
TGGTCGC93700.013.03915342
GGTCACT381550.012.6173157
Produced by FastQC (version 0.11.5)