FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45801734
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1251340.27320799688500874No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA557060.1216242162360054No Hit
CCGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATAG529720.11565500991731012No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG506870.11066611582871513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG382950.016.13575445
GGGAGGT561850.016.1078763
GGAGGTC555700.015.8947644
GGTCACT581950.014.956537
TGGGAGG426500.014.8999672
CTGGGAG464200.014.5863071
AGGTCAC612000.014.5382446
GTCACTC604650.014.4880668
GGCATAG432250.014.23304635
GAGGTCA635450.014.0546435
ATAGGGG440850.014.033565538
ACTCTCC629000.013.93793211
GAGCTTA442150.013.92440544
TGGCATA446000.013.86456534
CACCCGG157000.013.3966421
TCACTCT663950.013.33303459
CACTCTC657550.013.30196510
AGGCTCT500550.012.55556920
CTCCCCA510550.012.29445214
ACCCGGG172750.011.8497512
Produced by FastQC (version 0.11.5)