FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53120393
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG118100.020.2294465
ACCGCGT119200.020.1371296
AGACCGC120400.019.9364264
CGCGTTC130200.018.5559628
CAGACCG131500.018.5272983
CCGCGTT130650.018.474837
CGAACGA57850.017.69874632
ACGAACG61750.016.69002231
GAACGAT149050.016.45204445
CGCGTGA9650.015.6499941
CGTTACG15550.015.62368721
GTTCCTA88450.014.7509615
TCAGACC169450.014.6036192
CCCGTAT37950.014.314361
AACGAAC75100.013.87218430
GTCCGTT39750.013.836281
GCGTTCT176800.013.8050399
AGAACGA180000.013.70086544
CTCCGTT65500.013.5932241
CGCGTCG7350.013.493731
Produced by FastQC (version 0.11.5)