FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53120393
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG143150.012.43257538
AGCTTAG143450.012.10845245
GGCATAG143900.011.99251135
GGGAGGT204350.011.9244623
GTTGTCG27100.011.62333126
GGAGGTC210650.011.0871734
CATAGGG162900.010.63519937
GCATAGG167800.010.36485236
GAGCTTA168550.010.34534144
TGGCATA171700.010.15563234
ACTCTCC231950.010.01053211
GGTCACT227700.09.9009437
TTAACGG52600.09.5390273
GAGGTCA253850.09.3951445
TAGGGGA239500.09.33806439
TCACTCT267250.08.9997799
CACTCTC254400.08.95909710
CGCGGTA55800.08.74978511
GTCACTC282150.08.3738
CGTAGGG60550.08.36073237
Produced by FastQC (version 0.11.5)