FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45953166
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1532390.3334677745598638No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG634690.1381167077802648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC332600.023.917684
CAGACCG339100.023.5189443
GACCGCG338500.023.3345975
ACCGCGT341850.023.1388426
CGCGTTC353400.022.3769058
CCGCGTT355400.022.2793317
GAACGAT356800.022.10933145
TCAGACC382250.021.0641882
CTCAGAC414550.020.4062791
GCGTTCT393950.020.1307419
AGAACGA403750.019.56091944
TTCGGCG94150.019.04726844
AATACGG433750.018.22432
ATACGGA438150.018.00504333
TACGGAG436500.018.00093534
CGTTCTC449650.017.93224510
TCGGCGC102550.017.28936845
ACGGAGA455800.017.23905635
ACTCCCC469950.017.13456325
CAATACG464500.017.11799231
Produced by FastQC (version 0.11.5)