FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47955095
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT694840.14489388458098143No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG676700.14111117911454457No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG483150.10075050419564387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG161950.021.9078875
ACCGCGT162650.021.7443926
AGACCGC164650.021.5774444
CAGACCG172850.020.7289963
CCGCGTT177300.019.9352077
CGCGTTC181000.019.5024878
GCCGGCT216550.016.994881
TCAGACC219800.016.9260712
CCGGCTT259150.016.2666282
GCGTTCT220800.016.0275349
CTCAGAC265950.015.717031
TTCGACG30250.015.6929976
GAACGAT224800.015.61716345
CGAACGA43200.015.4703432
TCACGCT261600.015.38573558
GTTCCTA76800.015.05580815
TTCACGC269700.014.9989937
CCCAGAT194850.014.9714251
GTCCGTT37000.014.7830181
ACGCTCA280900.014.64068810
Produced by FastQC (version 0.11.5)