FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47955095
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA643250.1341359035989815No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG534970.11155644671332629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG156350.012.3696138
GGGAGGT256700.011.8142383
GGCATAG160300.011.71388535
GGAGGTC243600.011.70507054
AGCTTAG162600.011.51640445
GTTGTCG29000.010.71393726
GAGGTCA276600.010.6588365
ACTCTCC264450.010.65799911
GTCACTC270450.010.3627328
AGGGATC176850.010.3339442
AGGTCAC283050.010.2246276
GAGCTTA186650.010.1769644
TGGCATA185350.010.16324534
TCACTCT288100.010.0558769
CACCGAA347700.09.92319645
GGTCACT277850.09.8757997
CACTCTC293000.09.60420610
GCACCGA360650.09.54792844
ACCCGGC63500.09.4203535
TAGCGTA6050.09.30376137
Produced by FastQC (version 0.11.5)