FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44173536
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA703110.15916996094675326No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG504400.11418601399715883No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG483110.10936638624537551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT235550.013.7561533
GGAGGTC249750.012.9829714
ATAGGGG176600.012.51083538
GGTCACT253600.012.4297177
GGCATAG176700.012.40194735
AGCTTAG178500.012.31437945
GTTGTCG24450.012.23983826
AGGGATC174900.012.1707952
GTCACTC261500.012.0025878
TGGCATA196000.011.30690434
GAGCTTA194950.011.2637344
TCACTCT287150.011.1577359
CAGGGAT217100.010.5720321
CGCTCGC33600.010.24598125
TGGGAGG231650.010.1119772
GAGGTCA335550.010.1058225
CCTAGGC104400.09.9997678
CATAGGG224600.09.90723737
TATCCTA112150.09.8915265
AGCGTAT47950.09.85341645
Produced by FastQC (version 0.11.5)