FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48822550
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1566570.32087017167272086No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA548950.11243779769798998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT268550.028.509745
AGAACGA344450.022.2870944
AGACCGC352450.022.0662884
CAGACCG358750.021.7038673
GACCGCG391000.019.9309755
AATACGG401300.019.45388232
ATACGGA400950.019.4488133
TACGGAG401400.019.39897334
ACCGCGT407200.019.1988166
GCGTTCT409400.019.1929479
AAGAACG401700.019.1349243
CCGCGTT414950.018.9748127
CTCAGAC439700.018.8721621
CGCGTTC417900.018.8510158
TCAGACC426350.018.6319732
CAATACG420950.018.58207131
ACGGAGA426900.018.2507635
CCCAATA441650.017.801729
CGTTACG16500.017.45181321
CGGAGAG449850.017.3600936
Produced by FastQC (version 0.11.5)