FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48822550
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG23050.015.03231326
AGCTTAG146000.014.51674445
GGCATAG163350.012.90642135
ATAGGGG170650.012.60459738
ACCCGGC73900.012.6045425
GGGAGGT256000.012.3747143
GAGCTTA173400.012.23583844
ATAGCTC73650.011.54772439
CATAGGG186800.011.514853537
GGTCACT253550.011.4916217
ACTCTCC268050.011.02948311
GCATAGG195600.010.88197336
TGGCATA194950.010.8720934
GTCACTC275150.010.7448658
GGAGGTC296300.010.4258474
TCACCCG94100.010.4248393
TAGCTCT83000.010.38240640
CACTCTC295800.010.11646110
TGTCGTT34350.09.956182528
CACCCGG102950.09.7253694
Produced by FastQC (version 0.11.5)