FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49278569
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT707580.14358777341931336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT156550.021.24311645
AGACCGC158750.020.8051284
CAGACCG166700.019.9207153
GACCGCG169500.019.4059895
ACCGCGT178550.018.5476886
AGAACGA187600.017.751344
CCGCGTT189100.017.5128127
CGCGTTC191050.017.3576158
TTCGGCG66450.017.20164744
GTCCGTT43700.016.592141
CCGTACG71250.016.0412034
AATACGG211550.015.63515132
ATACGGA213750.015.54871933
AAGAACG214800.015.54579143
CGTACGC73100.015.5429025
TACGGAG214450.015.4460534
GCACTAC77800.015.26965325
TCGGCGC76350.014.91208445
TCAGACC226700.014.8766052
CAATACG228000.014.63499531
Produced by FastQC (version 0.11.5)