FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49278569
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG139550.015.75645845
ATAGGGG152950.014.22658838
GGCATAG155900.013.91390335
GAGCTTA169850.012.95859544
GGTCACT264900.012.6454627
GTCACTC270400.012.5962478
GGGAGGT282500.012.4397443
CATAGGG179050.012.19036837
TGGCATA183650.011.99520434
ACTCTCC289500.011.85070311
GCATAGG193850.011.37578636
AGCATAG104100.011.19883645
GGAGGTC311600.011.0975044
GCGTTCG32450.011.0930415
TAGGGGA313850.010.58249339
ATAGCTC56200.010.5303639
TCACTCT332650.010.52310859
GAGGTCA340850.010.4549355
CGTAGGG110100.010.21885137
CGCGTTC35800.010.18056814
Produced by FastQC (version 0.11.5)