FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47649727
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG685230.1438056507647987No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA584570.12268066089864481No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG533730.11201113492213713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG33900.017.123226
GGGAGGT303200.015.4532863
AGCTTAG224350.015.4044645
ATAGGGG241700.014.54947338
GGCATAG237350.014.52248235
GGAGGTC335750.013.9735714
GGTCACT332800.013.7451517
GTCACTC336600.013.5622428
GAGCTTA257450.013.54588544
ATATAGC51500.013.413196
TGGCATA267750.013.03327734
TCACTCT359750.012.9268669
TGGGAGG290800.012.7251632
GAGGTCA382500.012.5829015
TGTCGTT50200.012.14612728
ACTCTCC382700.012.09294611
TCGTTCC51000.011.646770530
GCTAAGT52150.011.5620414
CATAGGG306400.011.38924137
GCATAGG318950.010.969316536
Produced by FastQC (version 0.11.5)