FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46091287
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG29250.018.9975936
CGGTATA40650.018.15372520
CGTTACG15750.016.42745421
CGCAAAC65350.016.07779925
TTAGTTA70900.015.7384698
GTTCGAC35700.015.3772375
GAACGAT116100.015.30941745
CGACGAG36150.015.2470148
TCCGCAA69100.015.23782923
CCGCAAA72250.014.66690224
AGACCGC123600.014.4712914
GTCCGTT37550.014.2061561
TAGTTAC76400.014.1343199
CAGACCG129650.013.9521523
TCGACGA42300.013.5089137
AACGCTA105150.013.47989723
CGAGTCT79150.013.477241538
ATTTAGT90700.013.2948996
ACGTTCG9650.013.2884726
ACGCTAC107550.013.2000124
Produced by FastQC (version 0.11.5)