FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46091287
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG24050.011.97415626
AGCTTAG104700.010.81209645
ATAGGGG111100.010.35006538
GGCATAG109350.010.16573435
GTCACTC163800.09.8065658
GGTCACT166400.09.4910877
GGGAGGT188200.09.3482563
CATAGGG121800.09.1451537
CACTCGA107550.09.0786815
ACACTCG115050.08.9757244
GCATAGG126850.08.79880536
ACTCTCC184950.08.73400711
AACTCTA84550.08.65084245
GAGCTTA132800.08.54116844
GGCGTAG51500.08.47666635
TGGCATA136150.08.34603734
GCGTAGG51950.08.27332936
TGTCGTT37250.08.2141628
ATATAGC65000.08.2029216
ACTCGAG131700.08.1995326
Produced by FastQC (version 0.11.5)