FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50471269
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT977230.19362104804616662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT209500.022.1251245
AGACCGC245000.019.1447284
CAGACCG257000.018.3560333
AGAACGA257750.018.03108844
GACCGCG266900.017.4893995
TTCGACG22500.016.996396
ACCGCGT276900.016.9709366
CGCGTTC284000.016.5624228
CCGCGTT284600.016.5196027
CCGTACG70500.015.6675694
AAGAACG300400.015.47108143
TCAGACC311400.015.4455232
CGTTACG18800.015.44052321
GCGTTCT307000.015.3948639
ATACGGA303300.015.37606533
TACGGAG305050.015.21308334
AATACGG307450.015.20609532
CGTACGC71700.015.1855885
CTCAGAC345400.015.0113881
CCCGTAT33750.014.8693441
Produced by FastQC (version 0.11.5)