FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50471269
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG140100.013.45895645
ATAGGGG141950.013.37971138
GGCATAG145450.012.73279335
GGGAGGT243000.011.8734333
CATAGGG163450.011.55086637
GAGCTTA165550.011.471045544
GGTCACT238650.011.1086897
GCATAGG170400.010.98730836
GTCACTC244800.010.9857098
TGGCATA172650.010.96028934
GGAGGTC268000.010.5294244
ACTCTCC260050.010.40413111
CGTAGGG77950.09.90183237
TCAGACG82300.09.8726522
GCGTAGG79450.09.57327136
GGCGTAG79950.09.54154635
GTTGTCG20750.09.4328226
AGGTCAC300000.09.429066
CACTCTC295250.09.26346610
TCACTCT304100.09.232679
Produced by FastQC (version 0.11.5)