FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45633693
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1098410.2407015360339125No Hit
CCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCCAGCACGG582860.1277258011969358No Hit
CTGGGCTTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTG546020.1196528188064902No Hit
CTTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGG540150.11836648855046643No Hit
CCTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAGG497380.10899402772420808No Hit
CTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTC497270.10896992272792824No Hit
GTCCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCCAGCAC470240.10304666773298404No Hit
GTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTTCCC458380.10044771086135851No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT187300.028.78155745
GACCGCG207350.025.9595175
AGACCGC208900.025.820764
ACCGCGT209100.025.6953226
AGAACGA212300.025.37110144
CGCGTTC216100.024.8732368
CCGCGTT216550.024.8113217
CAGACCG222300.024.4060333
ATACGGA244550.022.13564533
AATACGG243950.022.11662132
AAGAACG245150.021.97143643
TACGGAG249000.021.7309934
GCGTTCT250600.021.5297539
TCAGACC255200.021.4738832
CAATACG262150.020.61548631
ACGGAGA264350.020.44360535
CGGAGAG269650.019.98337636
CCCAATA276450.019.5735229
TTCGGCG63750.019.09335744
CTCAGAC310050.018.9492071
Produced by FastQC (version 0.11.5)