FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51056734
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2066400.40472624042109706No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1801900.35292112495875666No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG681480.1334750475813827No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT641090.12556423996881586No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA633680.12411291329367054No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG615240.12050124475255311No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC527200.10325768193476692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT397950.024.68492345
GCCGGCT387300.023.3340851
CCGGCTT470150.021.864652
ACCGCGT457200.021.7903146
CAGACCG459800.021.7891483
AGACCGC459750.021.7572634
GACCGCG460450.021.6508945
CCGCGTT472550.021.0866327
TTCGACG34300.020.9902656
CGCGTTC478600.020.8247768
TCACGCT497350.020.6141878
TTCACGC507950.020.210587
TCAGACC506700.019.9722212
AGAACGA498200.019.81278644
CGGCTTC542650.019.4658033
GCGTTCT525450.019.032259
CGTTACG28050.018.92944121
ACGCTCA562200.018.9010610
CTCAGAC602000.018.0735971
GTTCGAC37500.017.8789315
Produced by FastQC (version 0.11.5)