FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58388493
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2863410.49040656007340355No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC745760.12772379653641686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT441900.030.58034145
AGAACGA518800.026.21232244
ACCGCGT527900.025.8628086
GACCGCG527300.025.8409655
AGACCGC532650.025.6149234
CCGCGTT538550.025.376437
CGCGTTC539300.025.3327968
CAGACCG555100.024.6884123
TCAGACC590600.023.4866262
GCGTTCT594900.023.0860929
AATACGG596300.022.85806832
AAGAACG594900.022.76843343
ATACGGA606350.022.52744333
TACGGAG608000.022.39973434
CTCAGAC660100.021.98991
CAATACG630300.021.67502231
ACGGAGA636550.021.50119435
CGGAGAG644700.021.1876736
CCCAATA662450.020.6909829
CGTTCTC694550.020.07834610
Produced by FastQC (version 0.11.5)