FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44900822
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT466070.10379988143646904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT114100.018.89654745
AGACCGC121050.017.8967024
GACCGCG127250.017.0070425
CAGACCG130550.016.7322143
ACCGCGT131100.016.5248876
CCGCGTT135250.016.0012577
CGCGTTC135500.015.9717368
CCCGTAT35450.015.3683421
AGAACGA142000.015.2317344
GTCCGTT38200.014.8513211
CGTTACG19850.014.39311321
GTTCCTA89600.014.28615215
CCCCCCT38250.013.89021
TACGGAG155600.013.84231734
CTCCGGT60650.013.8082951
CCTACTC94250.013.79615318
ATACGGA156700.013.78768333
AAGAACG159950.013.62088543
CTCCGAT46850.013.5508781
AATACGG160300.013.477532
Produced by FastQC (version 0.11.5)