FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53011151
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT674540.1272449262608918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC169350.019.056734
CAGACCG176100.018.4540733
GACCGCG183600.017.4795425
GAACGAT184650.017.45757145
ACCGCGT192650.016.7968396
CCGCGTT202100.015.9891677
CGCGTTC203150.015.8711538
CCCGTAT35650.015.4664621
CGTTACG15550.015.04669421
CCCGGAT131800.014.8896851
GCGTTCT219900.014.6724639
AGAACGA221300.014.60719444
GTCCGTT35100.014.2982261
TCAGACC241350.013.9591482
TACGGAG235050.013.73675934
CTCAGAC274600.013.6621641
AATACGG242850.013.31374532
ATACGGA244000.013.26049133
CAATACG246150.013.1992431
CGTACGC40950.013.13491255
Produced by FastQC (version 0.11.5)