FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53011151
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG138900.011.77553238
GTTGTCG20500.011.74327826
AGCTTAG142550.011.41081245
GGCATAG148900.011.01491635
GGGAGGT227500.010.8930043
GAGCTTA163700.010.0602144
CATAGGG170300.09.71004137
GGAGGTC255100.09.5380474
TGGCATA178900.09.243237534
GTCAGGA197150.09.1343491
GCATAGG182800.09.05836936
GGTCACT268900.08.5103577
GTCACTC280900.08.3070088
ATATAGC41200.08.1396746
CCGCTCA171400.08.07261630
CGTGGCG96000.08.0662491
CGTAGGG77200.07.78112337
ATAACGC49000.07.759593539
TAGGGGA289650.07.713013639
AGGGGAG300300.07.701691640
Produced by FastQC (version 0.11.5)