FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42636799
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1306370.30639495239781017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT295900.026.2041551
CCGGCTT358350.024.4208772
TTCACGC385900.022.4681977
TCACGCT385300.022.398118
TTCGACG22200.022.2940396
CGGCTTC401200.022.2107373
ACGCTCA408500.021.3738810
CACGCTC429000.020.2319039
AACGCTA425550.019.85081723
ACGCTAC427800.019.72080424
CGCTACC437300.019.37093725
AAAACGC444200.019.08786421
GAAAACG446250.018.83583520
CGTTACG21250.018.7430121
AAACGCT453950.018.6830922
AGACCGC140300.018.4399284
GACCGCG140050.018.3925295
ACCGCGT143300.018.1009836
CGCTCAG492900.017.69613611
GGCTTCA524300.017.5708624
Produced by FastQC (version 0.11.5)