FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42636799
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG658160.15436430863395725No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA572210.1342056658615484No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG444960.10436055483433453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG27350.012.33999126
GGGAGGT261550.012.2844123
GGAGGTC254500.012.2003484
TCAGACG77800.011.4813352
AGCTTAG208250.011.45314345
GGCATAG217000.010.86633235
ATAGGGG224900.010.86480238
AGGGATC167900.010.8278462
CGTGGCG127800.010.8098331
TGTCGTT35100.010.25637228
GAGCTTA234400.010.12741844
CCGTACG10800.09.9999634
TGGCATA238850.09.87228134
ACTCTCC308050.09.83846411
GGTCACT305900.09.8340487
GTCACTC316650.09.6138818
TGGGAGG267150.09.6013232
CAGGGAT208500.09.5827091
CAGACGT91300.09.5618493
ATATAGC41650.09.3997146
Produced by FastQC (version 0.11.5)