FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48957767
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT617090.12604537294358217No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG616340.12589217968213298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC156600.020.5667084
ACCGCGT161050.019.9599116
GACCGCG160650.019.935715
CAGACCG167300.019.2925873
TTCACGC269150.018.9555237
CCGCGTT172100.018.7044897
TCACGCT273700.018.5417798
CCGGCTT213300.018.3398692
CGCGTTC180050.017.8910968
TTCGACG14500.017.530636
ACGCTCA303550.017.0815710
GCCGGCT198800.017.0769081
AACGCTA310900.015.9832523
ACGCTAC313900.015.93210624
CACGCTC324750.015.8140769
TCAGACC211650.015.739132
CGCTACC323450.015.41316725
CGGCTTC270050.015.1359263
AAAACGC331850.015.07476921
GCGTTCT216500.014.9724599
Produced by FastQC (version 0.11.5)