FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55150064
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACATA1240430.22491904995794745No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG705090.1278493529944045No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT679950.12329088140314759No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA589630.10691374718984914No Hit
GAAAAAAATTCCAAAAGAGAACCACACACTACCATATCCAAACAACTTTTG578220.10484484659890876No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC572080.10373152060168055No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG562620.1020162007427589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG42650.025.0016946
TCGACGA50600.021.5171597
GTTCGAC48650.021.2246235
CGGTATA71200.020.56795120
CTATCCC115350.019.18967217
AGTGTAC116450.019.12431111
CGTTACC37700.018.55835245
GACCGCG170750.018.550355
ACCGCGT172400.018.3989056
AGACCGC173400.018.3446944
TACTATC120950.018.2639915
TGTACTA122100.017.90773613
ACTATCC122550.017.8786916
TCGTTAC40750.017.66964544
CAGACCG179750.017.6590883
GTACTAT127900.017.39465314
CCGGCTT266000.017.1681922
GTCCGTT45400.017.1304551
CCCTAAC48800.016.8146291
CGTTACG39200.016.75663621
Produced by FastQC (version 0.11.5)