FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50111687
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA586580.11705453061278898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG141900.013.3497538
GGGAGGT223400.012.4617033
AGCTTAG157050.012.03071545
GGAGGTC227250.011.7858594
GGCATAG158000.011.73417635
GAGCTTA177450.010.8001544
TGGCATA182600.010.38746834
GTCACTC251700.010.1831788
CATAGGG183900.010.11835237
GGTCACT267500.09.6739897
AGGGATC168350.09.6164662
CACCGAA320300.09.24866345
GCATAGG204150.09.13682836
GTTGTCG17350.09.08069526
TAGGGGA283950.08.95276939
TGGGAGG235850.08.9425842
GCACCGA330050.08.92807344
ACTCTCC295700.08.71494611
TCACTCT306850.08.6687569
TCTCCCC298100.08.60625813
Produced by FastQC (version 0.11.5)